smooth muscle myosin heavy chain (SM-MHC) and alpha smooth muscle actin (-SMA). Abstract Interstitial cells in the scars of human myocardial infarctions of different postinfarction times (6 hours to 17 years old) were characterized by antibodies to alpha-smooth muscle actin (ASMA), vimentin, and desmin. PMID: 9566278 . raised against avian gizzard actin. This protein is reported to have a . In addition, it has also been shown that smooth muscle actin appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases. 12 - 14 Blood flow is regulated by key elements of the vascular apparatus . DOI: 10.1002/path.1311 Abstract We investigated the distribution of alpha-skeletal, alpha-cardiac, and alpha-smooth muscle actin isoforms in human heart during development, hypertrophy, and failure. The repair of cutaneous wounds in the postnatal animal is associated with the development of scar tissue. Alpha smooth muscle actin antibody (ab5694) stains smooth muscle cells in vessel walls, gut wall, and myometrium. Computer-aided image analysis was performed for morphometrical . Methodology Immunohistochemistry Performed Monday - Friday Turnaround 2 - 3 business days Specimen Requirements For Ventana IHC, sections should be on Plus(+) charged slides. Immunostains for alpha-smooth muscle actin demonstrated a prominent pericytic component in several tumors (24%). The human protein, encoded by the gene ACTA2, is 377 amino acid residues long and has a mass of 42,009 daltons. alpha smooth muscle Actin ELISA Kits. Defects in this gene cause aortic aneurysm familial thoracic type 6. Smooth muscle actin expression in pilar leiomyoma. Myoepithelial cells in breast and salivary gland are also stained. These antibodies target Alpha-Smooth Muscle Actin in Human, Mouse, Rat, Chicken and Non-human primate samples. Over the lifetime, 1130 publication(s) have been published in the journal receiving 43000 citation(s). Hide. | Find, read and cite all the research you need on . Smooth muscle alpha-actin (SMA) is the definitive marker for the activated cardiac fibroblast population known as the myofibroblast ( Frangogiannis et al., 2000 ), but this actin isoform is also abundant in vascular smooth muscle cells ( Skalli et al., 1989 ). Match Criteria: Product Name, Keyword. In this study, we explored the possibility that modulation and replication of such septal fibroblasts result in the appearance of alpha-smooth muscle (alpha-SM) actin-positive myofibroblasts, typical of lung fibrosis. Basal lamina deposition was studied with antibodies to the basal lamina protein type IV collagen. Explore 27 research articles published in the Journal Seminars in Diagnostic Pathology in the year 1998. Alpha-smooth muscle actin (alpha-SMA) is the actin isoform that predominates within vascular smooth-muscle cells and plays an important role in fibrogenesis. It is a useful aid for classification of leiomyomas, leiomyosarcomas (1), and pleomorphic adenomas (2). This actin is an alpha actin that is found in skeletal muscle. . Immunogen N-terminal synthetic decapeptide of -smooth muscle actin. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. Immunostaining scores of glypican-3 and alpha-smooth muscle actin were significantly increased in biliary atresia livers. Histological analysis uncovered a marked reduction in lesions in LBP / transplanted mice, with decreased staining for the macrophage-specific marker CD68, as well as -smooth muscle actin (Fig. It reacts specifically with -smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections. Multiple alternatively spliced variants . Monoclonal Mouse Anti-Human. However, the . An increase in alpha-SMA expression up to 40% of the total cell population was seen when pericytes were treated with transforming growth factor-beta 1, whereas basic fibroblast growth factor slightly inhibited alpha-SMA expression. 6.1 Benign; . Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. 5C). This actin is an alpha actin that is found in smooth muscle. In humans, three main actin groups have been identified - alpha, beta, and gamma. Smooth muscle alpha-actin (SMA) is the definitive marker for the activated cardiac fibroblast population known as the myofibroblast ( Frangogiannis et al., 2000 ), but this actin isoform is also abundant in vascular smooth muscle cells ( Skalli et al., 1989 ). IHC Primary Antibodies . In this study, we investigated ASMA-positive stromal cells and their relation to tumor fibrosis in 50 cholangiocarcinomas, 30 hepatocellular carcinomas, and 57 metastatic liver carcinomas. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. Actin (Smooth Muscle) (Concentrate) Clone 1A4. Virchows Arch A Pathol Anat Histopathol. Normally, your immune system makes antibodies to attack foreign substances like viruses and bacteria. Clonality Application Species Reactivity Citations SDS; SAB5600065: RM253, monoclonal recombinant monoclonal: IHC: human: Expand. NET-containing histone H4 causes smooth muscle cell lysis and death provoking fibrous cap . The journal publishes majorly in the area(s): Lymphoma & Sarcoma. . Actin is a 43000 kDa ubiquitous protein found in all cells Actins are involved in cell motility (alpha, smooth muscle) and the maintenance of the cytoskeleton (beta and gamma, all cells) Antibodies to alpha smooth muscle actin do not detect the other actin isoforms Essential features Involved in cell motility DOI: 10.1177/37.3.2918221 Abstract alpha-Smooth muscle (alpha-sm) actin, an isoform typical of smooth muscle cells (SMC) and present in high amounts in vascular SMC, was demonstrated in the cytoplasm of pericytes of various rat and human organs by means of immunocytochemistry at the electron microscopic level. An autoantibody attacks the body's own cells and tissues by mistake. Storage/Transport Conditions Room temperature (use cold packs for transport of blocks during summer months) CPT Code(s) 88342 Requisition Synonym(s): alpha-Actin-2, human alpha smooth muscle Actin. Fifty-five percent of the tumors had intracytoplasmic aggregates of laminin. Definition / general Benign tumor composed of oncocytes Also called oxyphilic adenoma 1 - 2% of salivary gland neoplasms; more frequent than oncocytosis and oncocytic carcinoma Clinical features Usually parotid gland mass, also submandibular gland or minor salivary glands Mean age 60 years 20% associated with radiation therapy or radiation exposure To evaluate whether -smooth muscle actin (-SMA) plays a role in fibroblast contractility, we first compared the contractile activity of rat subcutaneous fibroblasts (SCFs), expressing low levels of -SMA, with that of lung fibroblasts (LFs), expressing high levels of -SMA, with the use of silicone substrates of different stiffness degrees. Summary. . The isoforms are comprised of three alpha actins (skeletal, cardiac, smooth), one beta actin (beta-non-muscle) and two gamma actins (gamma smooth muscle and gamma non-muscle). Fifteen hypoplastic and 25 normal developed lungs were included in this study using monoclonal alpha-smooth muscle (sm) actin antibodies for smooth muscle detection. The antibody (also known as anti--Sm-1) is specific for the single isoform of -smooth muscle actin. Test Details Alpha smooth muscle isoforms, mtoepithelium etc. CD34 antigen was detected in 74% of cases, BNH9 in 72%, and cytokeratins in 35%. 1/9. This MAb is highly specific to actin from smooth muscles. Distinguishing hypertrophic scar (HS) from keloid histopathologically is sometimes difficult because thickened hyalinized collagen (keloidal collagen), the hallmark of keloid, is not always detectable and alpha-smooth muscle actin (alpha-SMA), a differentiating marker of HS, is variably expressed in both forms of scar. Directing cell activities to efficiently heal wounds while minimizing the development of scar tissue is a major goal of wound management and the focus of intensive research efforts. Actin is a major component of the cytoskeleton and is present in most cell types. Environmental Research and Public Health. The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. Although -SMA expression is a hallmark of the mature myofibroblast, its role in regulation of fibroblast behavior and function remains poorly understood. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. Actin: an essential player in cell adhesion and migration. Recently Viewed SMA (Alpha Smooth Muscle Actin) Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells. 24 calponin, a 34-kd polypeptide, modulates actomyosin adenosine triphosphatase (atpase) activity in the SMA: clone clone sm-1. The aim of this study was to examine which epithelial and stromal markers are predictive of histologically diagnosed ameloblastic carcinoma and can sufficiently differentiate it from solid/multicystic ameloblastoma (SA). Anti-Smooth Muscle Actin Antibody (B4) is a mouse monoclonal IgG 1 Smooth Muscle Actin antibody, cited in 213 publications, provided at 200 g/ml. SMA (Alpha Smooth Muscle Actin) is recommended for the detection of specific antigens of interest in normal and neoplastic tissues, as an adjunct to conventional histopathology using non-immunologic histochemical stains. Compare Product No. Oxygen consumption in the retina, per gram tissue, is greater than that in most other organs in the human body. We have studied the expression of alpha-smooth muscle actin, smooth muscle myosin, and desmin in granulation tissue during the healing of an open wound in the rat by means of electron microscopy and immunohistochemistry, at the light and electron microscopic levels, using specific antibodies directed against these proteins. [provided by RefSeq, Feb 2021] Acta2 actin alpha 2, smooth muscle, aorta [ (house mouse)] Gene ID: 11475, updated on 11-Oct-2022. An immunohistochemical study of the breast using antibodies to basal and luminal keratins, alpha-smooth muscle actin, vimentin, collagen IV and laminin. Its epitope lies in the first four N-terminal amino acids. ab124964 staining alpha smooth muscle Actin in SV40LT-SMC cells (positive control, top panel) and A431 cells (negative control, bottom panel). The use of vascular smooth muscle cell markers, e.g. 11 Blood flow to various vascular layers of the macula are constantly fluctuating in order to maintain the dynamic balance between energy supply and demand. The presence of MFs has been reported in normal oral tissues and . Subsets of fibroblasts contribute to tissue fibrosis in multiple ways, including generating contractile force to activate integrin-bound, latent TGF and secreting excess amounts of collagens and other extracellular matrix proteins (ECM) that make up pathologic scar. NE degrades F-actin and translocates to the nucleus where it will partially cleave histones promoting chromatin decondensation. International Journal of. Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Listed are ELISA Kits for the detection of alpha smooth muscle Actin, an alias name of actin alpha 2, smooth muscle. This antibody labels smooth muscle cells, myofibroblasts and myoepithelial cells. Distinct cutaneous plaque-like proliferation of fibroblasts and myofibroblasts ( J Cutan Pathol 1992;19:85, Br J Dermatol 1993;129:69 ) Also called cutaneous myofibroma. Alpha-smooth muscle actin (SMA) is a prominent protein expressed on brain vessels, especially in cells located closer to the arteriole end of the capillaries, which possibly influences the blood vessel contraction. Product Details. Anti-Smooth Muscle Actin Antibody (B4) is recommended for detection of enteric smooth muscle -Actin and, to a lesser extent, -smooth muscle, -skeletal . Note cytoplasmic staining of the muscularis mucosa, vascular walls and smooth muscle fibers in the lamina propria. 1 Institute of Pediatric Pathology and Placentology, Virchow-Klinikum, Berlin, Germany. reduced contraction by -sma-overexpressing cells was associated with attenuated proliferative activity, in the absence of any effects on apoptosis. jurnal BM 7 - Read online for free. Pathology Resident Wiki is a FANDOM Lifestyle Community. Actin is an important contractile protein, and is the principal component of the thin filament. Alpha-Smooth Muscle Actin Antibody (1A4/asm-1) is made to the N-Terminal decapeptide of Alpha smooth muscle isoform of actin and conjugated to KLH. Alpha smooth muscle actin-containing myofibroblasts persist, but their immunoreactivity appears reduced compared with day 7. These cells play a key role in physiologic and pathologic processes like wound healing and tumorigenesis. View Mobile Site All Photos (2) Anti-Actin-2, N-Terminal antibody produced in rabbit. Aims/methods: In the human liver, alpha-smooth muscle actin (ASMA) is present in smooth muscle of the vasculature, perisinusoidal cells (Ito cells), and myofibroblasts derived from perisinusoidal cells. Intersecting fascicles will often create halo effect around nuclei cut on cross section Should express multiple smooth muscle markers such as desmin, caldesmon, actin - alpha smooth muscle Actin - alpha smooth muscle expression by itself is not enough to call leiomyosarcoma Sarcomatoid squamous cell carcinoma : Found in all eukaryotic cells, actin is a protein essential to maintain cell structure. catalytic activity ATP + H2O = ADP + H+ + phosphate By similarity Source: Rhea 13065 Hide Rhea reaction ATP Alpha-Smooth Muscle Actin Antibodies Antibodies that detect Alpha-Smooth Muscle Actin can be used in several scientific applications, including Immunohistochemistry, Western Blot, Immunocytochemistry, Flow Cytometry and ELISA. SMAs attack smooth muscle tissues in the liver and other parts of the body. Alpha-Actin Actin, a polypeptide molecule, is one of the largest and most abundant proteins found in striated muscle tissue (nearly 20% of all cellular protein). -sma may be implicated in contraction and remodeling of the extracellular matrix, but is not sufficient to induce contraction. SMA (Alpha Smooth Muscle Actin) is recommended for the detection of specific antigens of interest in normal and neoplastic tissues, as an adjunct to conventional histopathology using non-immunologic histochemical stains. For more information about this format, please see the Archive Torrents collection. Women > men, usually age 20 - 39, but occasionally in children ( J Cutan Pathol 2011;38;967 ) Upper arms, neck or trunk. -sma expression may modulate cellular functions, beyond its effects on SMA (Alpha Smooth Muscle Actin) Human small bowel: immunohistochemical staining for Alpha Smooth Muscle Actin. Although capillary pericytes had been known not to have -smooth muscle actin (SMA), a recent study revealed that a specific fixation method enabled the visualization of SMA along retinal capillarie Structural alterations of pericytes in microvessels are important features of diabetic retinopathy. The alpha actins are expressed primarily in skeletal, cardiac, and smooth muscle, while the remaining beta and gamma. calponin and smmhc are two antibodies that are more specific for mecs. 1992;421:323-330. This review outlines recent advances in understanding the mechanisms of NET release and functions in sterile disease. Case Report An Orthodontic Approach for Garre's Sclerosing Osteomyelitis of the Mandible Ioan Barbur 1 , Simion Bran 1, *, Mihaela Baciut 1 , Gabriel Armencea 1 , Alexandra Iulia Aghiorghiesei 2 , Tudor-Sergiu Suciu 1 , Adina Maria Barbur 3 , Horia Opris 1, * , Grigore Baciut 1 and . A smooth muscle antibody (SMA) is a type of antibody known as an autoantibody. At 20 weeks of fetal life, alpha-skeletal actin was localized in a small proportion of subendocardial and papillary muscle cardiomyocytes. Type III collagen (Col3), expressed in early granulation tissu Experimental pulmonary fibrosis was produced by a unique intratracheal instillation of bleomycin to 28 rats. 1 Synonyms; 2 Antibodies; 3 Technical Info; 4 Staining Pattern; 5 Expression in Normal Tissues; 6 Expression in Neoplastic Entities. Epithelial membrane antigen, S-100 protein, and HMB45 were not detected. ab5694 reacts with tumors arising from smooth muscles and myoepithelial cells. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Pediatric cases more often in neck. Contents. Myofibroblasts (MFs) are modified fibroblasts that express features of smooth muscle differentiation and were first observed in granulation tissue during wound healing. Taken together, these results indicate that macrophage LBP expression is a determinant of susceptibility to atherosclerosis in LDLR / . Myofibroblasts are specialized contractile fibroblasts that play a critical role in generating the contractile force responsible for wound closure and pathological contractures.1,2 These cells are characterized by the acquisition of a contractile phenotype and the expression of -smooth muscle actin (-SMA),3-5 which is correlated with generation of contractile force.6,7 An understanding . The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility . There is clear evidence that the blood-brain barrier is damaged in AD and that vessel function is impaired. $ 469.95 Products Downloads Products SMA-L-CE 1ml NCL-L-SMA In Stock Ships via Overnight Online-only price (details) $ 469.95 Fibrosis is a common pathological sequela of tissue injury or inflammation, and is a major cause of organ failure. Part II: Epitheliosis and ductal carcinoma in situ. [Cited in This Article: ] In particular, smooth muscle actin is an alpha actin that is found in skeletal muscle. Immunocytochemistry was performed on smooth muscle cells from bovine aortas using the monoclonal anti-ACTA2 antibody. Results: Up-regulation of glypican-3 mRNA expression was observed in biliary atresia livers, and its expression was positively associated with alpha-smooth muscle actin, -catenin, c-Myc, and cyclin D-1. Anti-Actin, -Smooth Muscle antibody, Mouse monoclonal has been used in immunofluorescence staining, immunohistochemistry, immunoblotting, immunocytochemistry and enzyme linked immunosorbent assay (ELISA). In the pleomorphic areas the expression of smooth muscle markers (desmin 10 of 28, muscle-specific actin 13 of 28, and alpha-smooth muscle actin 14 of 28) was significantly reduced, compared with that in leiomyosarcomatous fascicular area (desmin 18 of 28, muscle-specific actin 26 of 28, and alpha-smooth muscle actin 24 of 28). Myofibroblasts are. More than a million books are available now via BitTorrent. Outline the importance of improving collaboration and communication amongst the inter-professional team to enhance early and accurate diagnosis for patients affected by congenital smooth muscle hamartoma and to avoid further evaluation and unnecessary medical intervention. The expression of alpha-SMA in vitro could be slightly up-regulated by incubation with serum-containing medium. 21-23 smmhc is a structural component (200 kd) unique to smooth muscle cells, which functions within the hexagonal array of the thick-thin filament contractile apparatus. Tested applications Suitable for: WB, IHC-P more details Species reactivity Studies using fibroblast-populated collagen lattices suggested that -SMA expression increases fibroblast contractile activity [33], and plays a crucial role in focal adhesion maturation [34]. It is a member of the Actin family. Explore soyepoxy7 Tumblr blog with no restrictions, modern design and the best experience - | Tumpik At 14 days, there is a large increase in stainable collagen with complex remodeling and reduplication of the collagen fiber network of the interstitium. We examined immunohistochemically Ki-67, epithelial membrane antigen (EMA), alpha-smooth muscle actin (alpha-SMA), calponin .

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