C. The approximate blood volume of a rat is 55-70 ml/kg of body weight. The guidelines are based on peer-reviewed publications1-9 and data and experience accumulated at NIH . For example, for a mouse weighing 25 grams, the maximum allowable blood collection may not exceed 0.25 grams or 0.25 ml. Each method requires anesthesia or restraint, depending on the area from which the blood is taken. 1. Scope . Share. Considerations a. This policy applies to all personnel collecting blood samples from laboratory rodents. Estimated CBV for laboratory rodent species are as follows: CBV for a 25gm mouse: 0.025 kg x 75 ml/kg = 1.9 ml Product Enquiry Managing Pain:Anesthesia and Analgesia Agent Guidelines. Submandibular Blood Collection in Mice IACUC Standard 0.25 to 0.5 mg/kg SQ or IM. Blood Collection. blood collection may result in hypovolemic shock, physiological stress and even. The animal is kept in a restrainer. This page includes the Animal Care and Use Policies and Guidelines for the use of animals in research at Emory University. The purpose of this guideline is to provide recommended blood sampling volumes and guidance on a variety of acceptable blood collection techniques in the anesthetized and conscious rodent. Policies Overview Animal User Requirements Policies by Committee. For rodents: A safe guideline for total maximum volume of blood to remove from a healthy animal is 1% of body weight (BWT) either as a single collection or as multiple collections over a 2 week period. General Information . Guidelines for Blood Collection: Rodents and Rabbits By Department of Laboratory Animal Resources 2015-09-11_18-36-05.jpg Pages: 7 Publisher: --Edition: 2011 Language: English ISBN: --Description: Overview on Blood Sampling; Collection Volumes; Terminal Collections; Anesthesia Methods; Training Available ; Blood Collection in Mice; Blood Collection in Rats; Blood Collection in Rabbits; Retro . Show abstract. Microvette CB 300 Blood Collection System Starting at $102. In addition, human platelet-rich plasma (PRP) prepared from blood collected into trisodium citrate (3.8% w/v) has a depressed ionic calcium concentration, which can cause platelet aggregation and release of substances . RBC life span of the rat is 42-65 days7. 3. Various regulatory agencies and guidelines have restricted the use of animals and the techniques used for blood collection in laboratory animals. Using a . View STD PROCEDURE - Misc Rodent Proceedures - SubMandibular Blood Collection Mice.pdf from BIOL 3520 at University of North Texas. It may be used to collect a small to medium volume of blood. RODENT BLOOD COLLECTION 6 Mice & Rats - Tail Vein Nick. Blood collection volumes The total blood volume in an adult mammalian laboratory animal species is approximately 7% of body weight. A personal licence is also required for the person taking the blood sample. II. Download PDF (629.65 KB) Accessibility- We aim to provide documents in an accessible format. The above guideline for acute or single blood withdrawal still holds at any given time point within this 14-day period. However, severe injuries may occur to the animal if this procedure is not done properly, and available alternatives should be used whenever possible. Microvette 100 Capillary Blood Collection with Conical Bottom Inner Tube, Serum or Li HEP Starting at $103. demonstrates the use of the tail vein for blood collection. The Tail Vein is located on the side of the tail. blood samples for cp evaluation (hematology, coagulation, and biochemistry) must be obtained from all animals at study termination for repeat-dose studies and may also be collected at interim time points for subchronic and chronic studies. 1 2 Capillary Blood Collection Tubes for PinPorts Sarstedt's capillary tubes streamline the collection of small blood samples. 1. A C18 column was used as stationary phase and the mobile . Rodent Blood Collection Surgery. A rat that has been trained to undergo tail vein blood collection. This is a non-survival (terminal) procedure. Rodent (Mouse and Rat) Procedures b. Guidelines for Survival Bleeding of Mice and The guidelines are based on peer-reviewed publications Lateral Tail Vein or Ventral/Dorsal Artery Sampling: A sterile 23- to 29-gauge needle or a buttery catheter Rodent capillary or pipette blood collection is used for rodent blood collection. Using clippers, remove the hair from the ear. Blood was collected from chloroform anaesthetized rats by cardiac puncture into plain bottles, centrifuged at 3000 revolutions/min (3000 rpm) for 10 min (Kumar et al., 2017 . General guidelines: The acceptable quantity and frequency of blood sampling is determined by the circulating blood volume and the red blood cell (RBC) turnover rate. For mice and rats, the circulating blood volume is approximately 55-70 ml/kg of body weight (1,2). Blood Collection: The Mouse. Circulating blood volume (CBV) should be determined from species-specific volume and weight data. A precise, careful introduction of the needle is always best. death . Maximum Recommended Injection volumes and needle size. Print. Non-terminal blood collection without additional monitoring (see below) should be limited to 10% of the total circulating blood volume on a single collection or every 2 week period for serial collections. . Rodent Anesthesia Guidelines. Routes and Volumes of Administration in Mice. Login Contacts. Maintaining Animal Study Areas (Rodents) Maintenance of Euthanasia Equipment. The purpose of this guideline is to provide recommended blood sampling volumes and guidance on a variety of acceptable blood collection techniques in the anesthetized and conscious rodent. Carefully warm animal with heat lamp or disposable hand warmers. A finger should be placed at the blood sampling site for approximately . A. 2, 3 in mice, interim evaluation of cp endpoints requires additional animals, as nearly the total Animal Housing Study Areas (PDF) Rabbit and Guinea Pig Procedures 4. Purpose This document outlines the preferred methods for collecting blood from animals and blood collection volume and frequency limits. euthanasia agent for small animals when properly administered, its acceptability is predicated on a number of critical factors as described in the AVMA Guidelines for the Euthanasia of Animals. My Profile. Home; Publications and resources; Resource library; Blood Collection Practice Guidelines National Hand Hygiene Initiative. collecting more than four samples in a day (24-hour period) is not advisable. Document Actions Illustration of 5.0 mm length Genetic identification of research rodents is often accomplished through the use of PCR analysis of DNA extracted from tail or ear punch tissue. Guidelines for tail tissue collection: Deviations from PSU IACUC genotyping guidelines must be described in the PI's IACUC . Table 2: Summary of Rodent Blood Sampling Techniques Route General Anesthesia Required Speed and Efficiency Mouse Rat Sample Quality Mouse Rat Repeated Sampling Relative Volumes Obtainable Potential for Complications Species Comments Mandibular No +++ N/A +++ N/A Yes Medium to large Low Mouse Rapid, easy and repeated samples possible Saphenous No ++ ++ ++ ++ Yes Small to medium Low Rat, Mouse . Scope This policy applies to all personnel collecting blood samples from laboratory rodents. III. Blood collection for experimental purposes must comply with the investigator's Animal Care and Use Committee (ACUC) approved protocol, including approved . Aim: The assessment of efficacy should be paralleled with extensive pharmacokinetic parameters, and a valid bioanalytical method is a pre-condition for accurate plasma concentration. These guidelines are based on . I. The entire animal can be warmed with a carefully placed heat lamp for 5-10 minutes or by . ARAC Guidelines for Survival Bleeding of Rodents . . Guidelines for the Use of CO2 for Euthanasia of Rodents. Blood collection in the rodent requires microtechniques and special materials ().Microcollection tubes such as Microtainer (Becton Dickinson, Franklin Lakes, NJ) or the Microvette (Sarstedt, Numbrecht, Germany) are available untreated for serum collection or with an anticoagulant such as heparin or ethylenediamine tetra-acetic acid (EDTA) for plasma collections. Materials & methods: A simple, specific, rapid and sensitive LCMS/MS method has been developed for quantitative analysis of aprepitant in rat plasma. Standard Operating Procedure: Blood Collection in Rodents (Revised 10/26/2017) These guidelines have been developed to assist investigators and the Howard University Institutional Animal Care and Use Committee (IACUC) in their evaluation of investigator choice and application of survival rodent bleeding techniques. Following training and acclimatisation, the rat appears calm and cooperative and dosen't require restraint during the procedure. For optimal health, blood draws should be limited to the lower end of the range. View b2_blood_collection_in_mice_and_rats.pdf from MATHS 108 at Hansraj Jiwandas College Of Education. Home / Tools and Guides / Species General Data Species: General Species Information Cat Cattle Chicken Dog Gerbil Guinea Pig Hamster Mouse Rabbit Rat Sheep Swine. The approximate total blood volume of a rat is 55-70 ml/kg (mean 64ml/kg) of body weight. 4. In general, the more blood that is taken, the longer one must wait before the rodent can be sampled again. Rodent Blood Collection Guidelines The maximum volume of blood that one can take from a rodent at any one time varies with the desired frequency of sampling. A project licence should detail the site, volume and frequency of blood sampling. All non-terminal blood collection without replacement fluids is limited to 10% of the total circulating blood volume of a healthy animal during a 2 week period. This article deals with the approved blood. Factors to consider when choosing the blood withdrawal technique: species of rodent to be bled size/weight of the animal to be bled quality of the sample required (sterility, tissue fluid contamination, etc.) Guidelines for Blood Collection in Mice and Rats. 2. Normative Data for the Cat General Information Adult Weight Male: 9-14 lbs . B. DNA may also be obtained from other samples, such as hair, blood and oral swabs. The guidelines are based on peer-reviewed publications as well as on data and experience accumulated at the Frederick National Lab. The methods of blood collection in mice can have varying effects on the animals. Guidelines for Rodent Survival Blood Collection Reviewed and Approved by FAU IACUC Effective Date: 04 December 2015 Last Reviewed/Revised: 29 January 2021 Mouse blood volume is normally 72 ml/kg body weight (0.072ml /g body weight) - assuming the animal is mature, healthy, and on an adequate plane of nutrition. Blood collection techniques can only be observed and not practised before applying for a personal licence. Volume: On average, the total circulating blood volume is equal to 5.5 -8.0 % of the animal's body weight. Policies, Guidelines, Exemptions. The acceptable quantity and frequency of blood sampling is determined by the circulating blood volume and the red blood cell (RBC) turnover rate. Blood Collection Practice Guidelines. Adoption (PDF) Adverse Events Report Form (PDF) Anesthesia and Analgesia (PDF) Updated October 2022. Guidelines for the Review of Animal Care and Use Protocols. These guidelines have been developed to assist investigators and the Howard University Institutional Animal Care and Use Committee (IACUC) in their choice and application of survival rodent bleeding techniques. Clean withdrawal site with alcohol. The guidelines are based on peer-reviewed . Excessive blood collection may result in hypovolemic shock, physiological stress and even death. A drop of blood can be 15-20l . In particular, the PRP preparation has a limited stability (no longer than 2 h) and contains plasma proteins, including enzymes. Non Rodent Mammal Anesthesia Guidelines. These should be justified in terms of the science and animal welfare. Describes procedures for the collection of blood from the heart as a terminal procedure in the mouse. The best results are obtained when administered 1015 minutes prior to blood - collection. Guidelines. b. Guidelines for Blood Collection in Mice and Rats Overview: These guidelines have been developed Handling Radio Active Animals. The CCAC guidelines: Miceprovides information for investigators, animal care committees, facility manag- ers, veterinarians, and animal care staff to help facilitate improvement in both the care given to mice and the manner in which experimental procedures are carried out. II. This procedure must be performed under deep general anesthesia. II. guidelines may be given, but only practice can be expected to provide any proficiency. On average, the total circulating blood volume is equal to 5.5 -7.0 % (~66 ml/kg) of the animal's body weight. Twitter; Facebook; Email; Downloads Blood Collection Practice Guidelines. Guidance General Information Policies . Blood flow should be stopped by applying finger pressure to the soft tissue. Blood Collection: The Rat. For survival blood collection, the frequency and quantity of sample collection is dependent on the circulating blood volume and the rate of red blood cell turnover of the animal and varies by species. Certain areas produce more blood during collection, and guidelines prescribe the total amount that can be drawn from a mouse via each method.. 1. Surgery Guidelines - Rodents. Microvette 100 Capillary Blood Collection with Round Bottom Inner Tube, EDTA $103. Retro-orbital blood collection in rodents can provide moderate to large amounts of blood when performed by well-trained personnel. To facilitate blood collection, warm the rodent first. B. Guidelines for Blood Collection in Mice and Rats Overview: These guidelines have been developed to assist investigators and National Institutes of Health (NIH) Institute/Center (IC) Animal Care and Use Committees (ACUC) in their choice and application of survival rodent bleeding techniques. Training 5. The bore of the nee- dle should be as large as possible to ensure rapid blood Blood may be collected using capillary tubes or Pasteur withdrawal without collapsing the vein; it should be pipettes via a skin . The maximum that can be collected as a non-terminal blood collection without replacement fluids is 10% of the total circulating . References . The intracardiac puncture generally represents the most practical method of blood collection from small rodents when more than a . Place animal in an appropriate restraint device. Maximum blood volumes should be taken only from healthy animals. Log in menu . It is the responsibility of the investigator to use . When combined with Instech Vascular Access Buttons or PinPorts they can be part of a significant refinement in rodent blood collection. Guidance. Rodent Blood Collection Contact an RARC trainer for training or guidance on using these blood collection techniques. The needle is inserted parallel above the vessel and the tip directed into the lumen along with the longitudinal axis. If you're having problems using a document with your . The total blood volume for a 300 g rat Technique Example: Central Artery Place the rabbit in a rabbit restrainer. The maximum amount of blood that can be drawn from a rodent in any 2-week period may not exceed 1% of the animal's body weight. 1,5 The euthanasia method must be appropriate to the research goals, species and age of the animal, approved. A 1 to 1-1/2-inch needle ranging in size from 22gauge to 20- gauge is recommended for blood collection . blood extraction in live rats c. Please refer to the Guidelines for Injections in Rodents and Rabbits, Virginia Tech Office of the Overview: These guidelines have been developed to assist investigators and National Institutes of Health (NIH) Institute/Center (IC) Animal Care and Use Committees (ACUC) in their choice and application of survival rodent bleeding techniques. Guidelines for Blood Collection in Mice and Rats Overview: These guidelines have been developed to assist investigators and National Institutes of Health (NIH) Institute/Center (IC) Animal Care and Use Committees (ACUC) in their choice and application of survival rodent bleeding techniques. Pig bleeding For blood sampling a vacuum tube and a 20 gauge needle is used. Guidelines-for-Rodent-Blood-Withdrawal-and-Tail-Biopsy - Read online for free. Field Research and Teaching Guidelines. General Guidelines A. 1. Go to: PROCEDURE FOR DORSAL PEDAL VEIN BLOOD SAMPLE COLLECTION Requirements include animal (rat or mice), rodent handling gloves, cotton, capillary tube, 23G/27G needle and blood sample collection tubes. When using the tail veins or artery, you may dip the tail in warm water (45C).

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